Narrow junctions (
zonula occludens, tight junctions) seal off the adjacent cells, regulating substance passage through the cellular lamina and allowing the maintenance of cellular homeostasis.
Recombinant Rabbit Monoclonal Antibody-Purified; Thermo Fisher Scientific) and
zonula occludens-1 (ZO-1; diluted at 1:200; TJP1/ZO-1 rat anti-mouse monoclonal antibody; LSBio, Seattle, WA, USA) were reacted to the sections overnight at 4[degrees]C.
The cells were incubated overnight with primary antibody (
zonula occludens protein 1 [Zo-1] invitrogen, 330100 and cytokeratin 18 chemicon, MAB3234).
Antibodies to HIF-2[alpha], occludin, and
zonula occludens (ZO-1) were purchased from Invitrogen Life Technologies (USA) and GAPDH was purchased from Proteintech Group Inc.
PDZ (postsynaptic density 95, PSD-85; Discs large, Dlg;
Zonula occludens-1, ZO-1) domains are modular protein interaction domains - formerly known as Discs-large homology regions (DHRs) or GLGF repeats, after a conserved Gly-Leu-Gly-Phe sequence found within the domain--that play a role in protein targeting and protein complex assembly (73) The presence of HTLV-3 and 4 viruses in Cameroon, though not associated with any diseases, is depictive of their possible presence in other West and Central African countries.
Moreover, the mRNA levels of occludin and
zonula occludens-1, two epithelial tight junction proteins, were increased, demonstrating that carvacrol may improve the integrity of intestinal epithelia.
ATP depletion of tubular cells causes dissociation of the
zonula adherens and nuclear translocation of beta-catenin and LEF-1.
junctions (
zonula occludens) and the presence of well-organized
zonulaAdhesive junctions include the following: (1) plasma membrane structures called tight junctions (TJ) comprised of multiple proteins (e.g., claudins, occludins, junctional adhesion molecules (JAMs));
zonula occludens (ZO proteins, actin); (2) multiprotein subjacent adherens junctions (AJ) including cadherin and catenin proteins; and (3) desmosomes.
To avoid any unnecessary trauma to intraocular structures (i.e., the crystalline lens, iris, ciliary sulcus, and
zonula), pIOLs were left in this horizontal position.
Lu et al., "Neuronal connexin36 association with
zonula occludens-1 protein (ZO-1) in mouse brain and interaction with the first PDZ domain of ZO1," European Journal of Neuroscience, vol.